CCMB

CSIR - Centre for Cellular & Molecular Biology
Council of Scientific and Industrial Research

The Innovation Engine of India

Ministry of Science & Technology, Govt. of India

The People

Amitabha Chattopadhyay

Amitabha Chattopadhyay

CSIR Bhatnagar Fellow
+91-040-27192578
amit@ccmb.res.in

Research Interests

The major research interest of our group is centered on membrane and receptor biology using a variety of biophysical, biochemical and cell biological approaches. Fluorescence-based spectroscopic and microscopic approaches are extensively used in our laboratory. The research interest of our group can be broadly categorized as follows:

Membrane Organization and Dynamics using the Wavelength-Selective Fluorescence Approach.
Novel Organization of Cholesterol in Membranes at Low Concentrations: Effects of Curvature Stress and Membrane Thickness.
Organization, Dynamics and Lipid-protein Interactions of the Serotonin1A (5-HT1A) Receptor.
Monitoring Organization and Dynamics of the Serotonin1A (5-HT1A) Receptor in Live Cells utilizing Dynamic Fluorescence Microscopy (FRAP and FCS).
Role of Membrane Cholesterol in Leishmaniasis.

Selected Publications

Chakraborty, H., and Chattopadhyay, A. (2015) `Excitements and Challenges in GPCR Oligomerization: Molecular Insight from FRET` (invited review) ACS Chem. Neurosci. 6: 199-206.

Viswanathan, G., Jafurulla, M., Kumar, G.A., Raghunand, T.R., and Chattopadhyay, A. (2015) `Dissecting the Membrane Cholesterol Requirement for Mycobacterial Entry into Host Cells` Chem. Phys. Lipids 189: 19-27.

Chattopadhyay, A., and Haldar, S. (2014) `Dynamic Insight into Protein Structure utilizing Red Edge Excitation Shift` Acc. Chem. Res. 47: 12-19.

Ganguly, S., Clayton, A.H.A., and Chattopadhyay, A. (2011) `Organization of Higher Order Oligomers of the Serotonin1A Receptor Explored utilizing Homo-FRET in Live Cells` Biophys. J. 100: 361-368.

Shrivastava, S., Pucadyil, T.J., Paila, Y.D., Ganguly, S., and Chattopadhyay, A. (2010) `Chronic Cholesterol Depletion using Statin Impairs the Function and Dynamics of Human Serotonin1A Receptors` Biochemistry 49: 5426-5435.

Education & Experience

P.G:

Chemistry ; Indian Institute of Technology, Kanpur ; 1980

Ph.D:

A Novel Fluorescence Quenching Method for Measurement of Membrane Penetration Depth ; State University of New York at Stony Brook ; 1987

Post.Doc:

Lipid-protein Interaction in the Nicotinic Acetylcholine Receptor ; Department of Biochemistry & Biophysics, University of California, Davis, CA, USA ; 1987-1989

Team Members

team-members-pic
Ashwani Sharma
Ashwani Sharma

Junior Research Fellow

Junior Research Fellow

Subhashree S Sahu
Subhashree S Sahu

Junior Research Fellow

Junior Research Fellow

Publications

Title

Journal

Year

Editorial
The Journal of Membrane Biology
2024
Interplay of Cholesterol and Actin in Neurotransmitter GPCR Signaling: Insights from Chronic Cholesterol Depletion using Statin
ACS Chemical Neuroscience
2023
Palmitoylation of the Glucagon-like Peptide-1 Receptor Modulates Cholesterol Interactions at the Receptor-Lipid Microenvironment
The Journal of Physical Chemistry B
2023
Smith-Lemli-Opitz Syndrome: A Pathophysiological Manifestation of The Bloch Hypothesis
Frontiers in Molecular Biosciences
2023
Role of Cholesterol and its Biosynthetic Precursors on Membrane Organization and Dynamics: A Fluorescence Approach
The Journal of Membrane Biology
2023
Statin-induced Increase in Actin Polymerization Modulates GPCR Dynamics and Compartmentalization
Biophysical Journal
2023
Effect of Local Anesthetics on Dipole Potential of Different Phase Membranes: A Fluorescence Study
The Journal of Membrane Biology
2022
Membrane Dipole Potential: An Emerging Approach to Explore Membrane Organization and Function
The Journal of Physical Chemistry B
2022
Inositol phosphoryl transferase, Ipt1, is a critical determinant of azole resistance and virulence phenotypes in Candida glabrata
Journal of Fungi
2022
Integrity of the Actin Cytoskeleton of Host Macrophages is Necessary for Mycobacterial Entry
The Journal of Membrane Biology
2022
Cholesterol-dependent Dynamics of the Serotonin1A Receptor utilizing Single Particle Tracking: Analysis of Diffusion Modes
The Journal of Physical Chemistry B
2022
Lysine 101 in the CRAC Motif in Transmembrane Helix 2 Confers Cholesterol-induced Thermal Stability to the Serotonin1A Receptor
The Journal of Membrane Biology
2022
Synergistic and Competitive Lipid Interactions in the Serotonin1A Receptor Microenvironment
ACS Chemical Neuroscience
2022
Metabolic Depletion of Sphingolipids Inhibits Agonist-induced Endocytosis of the Serotonin1A Receptor
Traffic
2022
Comparative Analysis of Tryptophan Dynamics in Spectrin and its Constituent Domains: Insights from Fluorescence
The Journal of Physical Chemistry B
2022
Cholesterol in GPCR Structures: Prevalence and Relevance
The Journal of Membrane Biology
2022
Chronic Cholesterol Depletion Increases F-actin Levels and Induces Cytoskeletal Reorganization via a Dual Mechanism
The Journal of Lipid Research
2022
Effect of Hypoxia on the Function of the Human Serotonin1A Receptor
ACS Chemical Neuroscience
2022
Metabolic Depletion of Sphingolipids Does not Alter Cell Cycle Progression in Chinese Hamster Ovary Cells
Journal of Membrane Biology
2022
Cholesterol in GPCR Structures: Prevalence and Relevance
Journal of Membrane Biology
2022
Integrity of the Actin Cytoskeleton of Host Macrophages is Necessary for Mycobacterial Entry
Journal of Membrane Biology
2022
Cholesterol-dependent Endocytosis of GPCRs: Implications in Pathophysiology and Therapeutics
Biophysical Reviews
2021
Cholesterol-dependent Endocytosis of GPCRs: Implications in Pathophysiology and Therapeutics
Biophysical Reviews
2021
Structure, Dynamics and Lipid Interactions of Serotonin Receptors: Excitements and Challenges
Biophysical Reviews
2021
Metabolic Depletion of Sphingolipids Reduces Cell Surface Population of the Human Serotonin1A Receptor due to Impaired Trafficking
ACS Chemical Neuroscience
2021
Conformational Plasticity and Dynamic Interactions of the N-terminal Domain of Chemokine Receptor CXCR1
PLoS Computational Biology
2021
Environment-sensitive Fluorescence of 7-Nitrobenz-2-oxa-1,3-diazol-4-yl (NBD)-labeled Ligands for Serotonin Receptors
Molecules
2021
ack of Environmental Sensitivity of a Naturally Occurring Fluorescent Analog of Cholesterol
Journal of Fluorescence
2021
Molecular Sensor for Cholesterol in the Human Serotonin1A Receptor
Science Advances
2021
Lipid Headgroup Charge Controls Melittin Oligomerization in Membranes: Implication in Membrane Lysis
The Journal of Physical Chemistry B
2021
Cholesterol Footprint in High-Resolution Structures of Serotonin Receptors: Where are We Now and What Does It mean
Chemistry and Physics of Lipids
2021
Hydration Dynamics in Biological Membranes: Emerging Applications of Terahertz Spectroscopy
The Journal of Physical Chemistry Letters
2021
Late endosomal/lysosomal accumulation of a neurotransmitter receptor in a cellular model of Smith-Lemli-Opitz syndrome
Traffic
2021
Insights into Cellular Signaling from Membrane Dynamics
Archives of Biochemistry and Biophysics
2021
Membrane Cholesterol Regulates Endocytosis and Trafficking of the Serotonin1A Receptor: Insights from Acute Cholesterol Depletion
Biochim. Biophys. Acta (Molecular and Cell Biology of Lipids)
2021
Effect of Tertiary Amine Local Anesthetics on G Protein-coupled Receptor Lateral Diffusion and Actin Cytoskeletal Reorganization
Biochim. Biophys. Acta (Biomembranes)
2021
Statin-induced Chronic Cholesterol Depletion Switches GPCR Endocytosis and Trafficking: Insights from the Serotonin1A Receptor
ACS Chem. Neurosci.
2020
Structure, Dynamics and Lipid Interactions of Serotonin Receptors: Excitements and Challenges
Biophys. Rev.
2020
Differential Sensitivity of pHLIP to Ester and Ether Lipids
Chem. Phys. Lipids
2020
Cell Cycle Dependent Modulation of Membrane Dipole Potential and Neurotransmitter Receptor Activity: Role of Membrane Cholesterol
ACS Chem. Neurosci.
2020
Role of Cholesterol and its Immediate Biosynthetic Precursors in Membrane Dynamics and Heterogeneity: Implications for Health and Disease
J. Phys. Chem. B
2020
Selectivity in Agonist and Antagonist Binding of Serotonin1A Receptors via G-protein Coupling
Biochim. Biophys. Acta (Biomembranes)
2020
Cholesterol Interaction Motifs in G Protein-coupled Receptors: Slippery Hot Spots?
Wiley Interdiscip. Rev. Syst. Biol. Med.
2020
Membrane Electrostatics Sensed by Tryptophan Anchors in Hydrophobic Model Peptides Depends on Non-Aromatic Interfacial Amino Acids: Implications in Hydrophobic Mismatch
Faraday Discuss.
2020
Role of Cholesterol-mediated Effects in GPCR Heterodimers
Chem. Phys. Lipids
2020
Structural Stringency and Optimal Nature of Cholesterol Requirement in the Function of the Serotonin1A Receptor
J. Membr. Biol.
2020
Molecular Determinants of GPCR Oligomerization
In: GPCRs - Structure, Function, and Drug Discovery (Jastrzebska, B., and Park, P.S.-H., Eds.), Elsevier
2020
Molecular Evolution of a Collage of Cholesterol Interaction Motifs in Transmembrane Helix V of the Serotonin1A Receptor
Chem. Phys. Lipids
2020
Exploring Membrane Lipid and Protein Diffusion by FRAP
Analysis of Membrane Lipids (Prasad, R., and Singh, A., Eds.), Springer
2020
Role of Actin Cytoskeleton in Dynamics and Function of the Serotonin1A Receptor
Biophys. J.
2020
A Critical Analysis of Molecular Mechanisms Underlying Membrane Cholesterol Sensitivity of GPCRs
Adv. Exp. Med. Biol.
2019
Differential Effects of Simvastatin on Membrane Organization and Dynamics in Varying Phases
Chem. Phys. Lipids
2019
Leishmania donovani Internalizes into Host Cells via Caveolin-mediated Endocytosis
Sci. Rep.
2019
Exploring Endocytosis and Intracellular Trafficking of the Human Serotonin1A Receptor
Biochemistry
2019
Effect of Local Anesthetics on the Organization and Dynamics of Hippocampal Membranes: A Fluorescence Approach
J. Phys. Chem. B
2019
Extramembranous Regions in G Protein-coupled Receptors: Cinderella in Receptor Biology
J. Membr. Biol.
2019
Exploring Membrane Organization at Varying Spatiotemporal Resolutions Utilizing Fluorescence-based Approaches: Implications in Membrane Biology
Phys. Chem. Chem. Phys.
2019
A Collage of Cholesterol Interaction Motifs in the Serotonin1A Receptor: An Evolutionary Implication for Differential Cholesterol Interaction
Chem. Phys. Lipids
2019
Micellar Dipole Potential is Sensitive to Sphere-to-Rod Transition
Chem. Phys. Lipids 195: 34-38.
2016
Molecular Anatomy of An Ion Channel Explored utilizing Fluorescence Spectroscopy
Reviews in Fluorescence 2015 (Geddes, C.D., Ed.), Springer, Heidelberg pp. 353-367
2016
What Can Simulations Tell Us About GPCRs: Integrating the Scales
Methods Cell Biol. 132: 429-452.
2016
Molecular Rheology of Neuronal Membranes Explored using A Molecular Rotor: Implications for Receptor Function
Chem. Phys. Lipids 196: 69-75.
2016
Spatiotemporal Control of Transmembrane Proteins Through the Cytoskeleton: An Evolving Story
Biophys. J. 110: 1036–1037.
2016
Journal of Membrane Biology: Membranes in Disease Section
J. Membrane Biol. 249:9
2016
Effect of Local Anesthetics on the Organization and Dynamics in Membranes of Varying Phase: A Fluorescence Approach
Chem. Phys. Lipids 198: 21-27.
2016
Membrane Fluorescent Probes: Insights and Perspectives in Fluorescent Analogues of Biomolecular Building Blocks: Design and Applications
(Tor, Y. and Wilhelmsson, M., Eds.) John Wiley pp. 356-366.
2016
Cholesterol Modulates Bitter Taste Receptor Function
Biochim. Biophys. Acta (Biomembranes) 1858: 2081-2087.
2016
Statin-induced Chronic Cholesterol Depletion Inhibits Leishmania donovani Infection: Relevance of Optimum Host Membrane Cholesterol
Biochim. Biophys. Acta (Biomembranes) 1858: 2088-2096.
2016
The Membrane as the Gatekeeper of Infection: Cholesterol in Host-Pathogen Interaction
Chem. Phys. Lipids 199: 179-185
2016
The Membrane as the Gatekeeper of Infection: Cholesterol in Host-Pathogen Interaction
Chem. Phys. Lipids 199: 179-185
2016
Cholesterol-Dependent Thermotropic Behavior and Organization of Neuronal Membranes
Biochim. Biophys. Acta (Biomembranes) 1858: 2611-2616
2016
Novel Insights in Membrane Biology Utilizing Fluoresence Recovery After Photobleaching
Adv. Exp. Med. Biol. 842: 27-40.
2015
Role of Lipid-mediated Effects in Beta2-adrenergic receptor Dimerization
Adv. Exp. Med. Biol. 842: 247-261.
2015
Excitements and Challenges in GPCR Oligomerization: Molecular Insight from FRET
ACS Chem. Neurosci. 6: 199-206.
2015
Excitements and Challenges in GPCR Oligomerization: Molecular Insight from FRET
ACS Chem. Neurosci. 6: 199-206.
2015
Excitements and Challenges in GPCR Oligomerization: Molecular Insight from FRET
ACS Chem. Neurosci. 6: 199-206.
2015
Excitements and Challenges in GPCR Oligomerization: Molecular Insight from FRET
ACS Chem. Neurosci. 6: 199-206.
2015
Solubilization of G Protein-Coupled Receptors: A Convenient Strategy to Explore Lipid-Receptor Interaction
Methods Enzymol. 557: 117-134.
2015
Organization and Dynamics of Tryptophan Residues in Brain Spectrin: Novel Insight into Conformational Flexibility
J. Fluoresc. 25: 707–717.
2015
Cholesterol-induced Changes in Hippocampal Membranes Utilizing A Phase-sensitive Fluorescence Probe
Biochim. Biophys. Acta (Biomembranes) 1848: 1699-1705.
2015
Dissecting the Membrane Cholesterol Requirement for Mycobacterial Entry into Host Cells
Chem. Phys. Lipids 189: 19-27.
2015
Get Your kICS by Measuring Membrane Protein Dynamics
Biophys. J. 109: 1-2.
2015
Guest Editor, special issue - Lipid-protein Interaction
Biochim. Biophys. Acta (Biomembranes) 1848: 1727-1728.
2015
Molecular Dynamics Simulations of GPCR-Cholesterol Interaction: An Emerging Paradigm
Biochim. Biophys. Acta (Biomembranes) (invited review) 1848: 1775-1782.
2015
Dipolar Rearrangement During Micellization Explored using A Potential-sensitive Fluorescent Probe
Chem. Phys. Lipids 191: 91-95.
2015
Sphingolipids in the Function of G Protein-Coupled Receptors
Eur. J. Pharmacol. 763: 241–246.
2015
Differential Dynamics of the Serotonin1A Receptor in Membrane Bilayers of Varying Cholesterol Content Revealed by All Atom Molecular Dynamics Simulation
Mol. Membr. Biol. 32: 127–137.
2015
Depth-dependent Organization and Dynamics of Archaeal and Eukaryotic Membranes: Development of Membrane Anisotropy Gradient with Natural Evolution
Langmuir 31: 11591-11597.
2015
Stereospecific requirement of cholesterol in the function of the serotonin1A receptor
Biochim. Biophys. Acta (Biomembranes) 1838: 158-163.
2014
Ion Channel Stability of Gramicidin A in Lipid Bilayers: Effect of Hydrophobic Mismatch
Biochim. Biophys. Acta (Biomembranes) 1838: 328-338.
2014
Importance of Indole N-H Hydrogen Bonding in the Organization and Dynamics of Gramicidin Channels
Biochim. Biophys. Acta (Biomembranes) 1838: 419-428.
2014
Dynamic Insight into Protein Structure utilizing Red Edge Excitation Shift
Acc. Chem. Res. 47: 12-19.
2014
Functional characterization of rare variants in human dopamine receptor D4 gene by genotype-phenotype correlations
Neuroscience 262: 176-189.
2014
Taking Care of Bystander FRET in A Crowded Cell Membrane Environment
Biophys. J. 106: 1227-1228.
2014
Cholesterol Modulates the Dimer Interface of the Beta2-Adrenergic Receptor via Cholesterol Occupancy Sites
Biophys. J. 106: 1290-1300.
2014
Integrity of the Actin Cytoskeleton of Host Macrophages is Essential for Leishmania donovani Infection
Biochim. Biophys. Acta (Biomembranes) 1838: 2011-2018.
2014
Location, Dynamics and Solvent Relaxation of A Nile Red-based Phase-sensitive Fluorescent Membrane Probe
Chem. Phys. Lipids 183: 1-8
2014
Lipid Binding Specificity of Bovine alpha-Lactalbumin: A Multidimensional Approach
Biochim. Biophys. Acta (Biomembranes) 1838: 2078-2086
2014
Membrane Dipole Potential is Sensitive to Cholesterol Stereospecificity: Implications for Receptor Function
Chem. Phys. Lipids 184: 25–29.
2014
GPCRs: Lipid-Dependent Membrane Receptors That Act as Drug Targets
Adv. Biol. 2014: 143023.
2014
Membrane Lipids in the Function of Serotonin and Adrenergic Receptors
Curr. Med. Chem. 20: 47-55.
2013
Application of NBD-labeled lipids in Membrane and Cell Biology
Springer Series on Fluorescence (Mely, Y., Duportail, G., Eds.), Springer, Heidelberg, vol. 13, pp. 37-50.
2013
Application of Quantitative Fluorescence Microscopic approaches to Monitor Organization and Dynamics of the Serotonin1A Receptor
Springer Series on Fluorescence (Mely, Y., Duportail, G., Eds.), Springer, Heidelberg, vol. 13, pp. 417-437.
2013
Differential Effect of Sterols on Dipole Potential in Hippocampal Membranes: Implications for Receptor Function
Biochim. Biophys. Acta (Biomembranes) 1828: 917-923.
2013
Organization and Dynamics of the N-terminal Domain of Chemokine Receptor CXCR1 in Reverse Micelles: Effect of Graded Hydration
J. Phys. Chem. B 117: 1225-1233.
2013
Cholesterol Biosynthesis and Homeostasis in Regulation of the Cell Cycle
PLoS One 8: e58833.
2013
A Toolbox of Fluorescence Microscopic Approaches Reveals Dynamics and Assembly of a Membrane Associated Protein
Biophys. J. (New and Notable) 104: 1844-1845.
2013
Targeted liposomes to deliver DNA to cells expressing 5-HT receptors
Int. J. Pharm. 419: 347-354
2012
Hydration Dynamics of Probes and Peptides in Captivity
Reviews in Fluorescence 2010 (Geddes, C.D., Ed.), Springer, New York pp. 155-172
2012
Organization and Dynamics of Hippocampal Membranes in a Depth-dependent Manner: An Electron Spin Resonance Study.
J. Phys. Chem. B 116: 2999-3006.
2012
Differential Effect of Cholesterol and its Biosynthetic Precursors on Membrane Dipole Potential.
Biophysical Journal 102: 1561-1569.
2012
Structural Transition in Micelles: Novel Insight into Microenvironmental Changes in Polarity and Dynamics.
Chemistry and Physics of Lipids 165: 497-504
2012
Sphingolipid Binding Domain in the Serotonin1A Receptor.
Advances in Experimental Medicine and Biology 749: 279-293.
2012
Membrane Cholesterol: Implications in Receptor Function.
Cholesterol Regulation of Ion Channels and Receptors (Levitan, I. and Barrantes, F.J., Eds.), John Wiley, pp. 255-271.
2012
Role of Membrane Cholesterol in Leishmanial Infection.
Advances in Experimental Medicine and Biology 749: 201-213.
2012
Comparative Analysis of Calcium Spikes upon Activation of Serotonin1A and Purinergic Receptors
PLoS One 7: e51857
2012
Transbilayer Organization of Membrane Cholesterol at Low Concentrations: Implications in Health and Disease.
Biochimica et Biophysica Acta (Biomembranes) 1808: 19-25.
2011
Cholesterol Depletion Enhances Adrenergic Signaling in Cardiac Myocytes.
Biochimica et Biophysica Acta (Biomembranes) 1808: 461-465
2011
Molecular modeling of the human serotonin1A receptor: role of membrane cholesterol in ligand binding of the receptor
Molecular BioSystems, 7: 224-234
2011
Identification of cholesterol recognition amino acid consensus (CRAC) motif in G-protein coupled receptors
Biochemical and Biophysical Research Communications, 404: 569-573
2011
Organization of Higher Order Oligomers of the Serotonin1A Receptor Explored utilizing Homo-FRET in Live Cells
Biophysical Journal, 100: 361-368
2011
Fixation Alters Fluorescence Lifetime and Anisotropy of Cells Expressing EYFP-tagged Serotonin1A Receptor
Biochemical and Biophysical Research Communications, 405: 234-237
2011
Membrane Organization and Dynamics of the Serotonin1A Receptor in Live Cells
J. Neurochem. 116: 726-733
2011
Reorganization of the actin cytoskeleton upon G-protein coupled receptor signaling
Biochimica et Biophysica Acta 1808: 1921-1929
2011
Organization and Dynamics of Membrane Probes and Proteins Utilizing the Red Edge Excitation Shift
J. Phys. Chem. B, 115: 5693-5706
2011
Desmosterol Replaces Cholesterol for Ligand Binding Function of the Serotonin1A Receptor in Solubilized Hippocampal Membranes: Support for Nonannular Binding Sites for Cholesterol?
Biochimica et Biophysica Acta 1808: 2428-2434
2011
Metabolic Depletion of Sphingolipids Enhances the Mobility of the Human Serotonin1A Receptor
Biochemical and Biophysical Research Communications 411: 180-184
2011
Oligomerization of the Serotonin1A Receptor in Live Cells: A Time-Resolved Fluorescence Anisotropy Approach
J. Phys. Chem. B 115: 11439-11447
2011
A Novel Mechanism for an Old Drug: Amphotericin B in the Treatment of Visceral Leishmaniasis
Biochemical and Biophysical Research Communications 416: 7-12
2011
Identification of a red-emitting fluorescent ligand for in vitro visualization of human serotonin 5-HT1A receptors.
Bioorganic & Medicinal Chemistry Letters 20: 6628-6632
2010
Amphotericin B Inhibits Entry of Leishmania donovani into Primary Macrophages
Biochem. Biophys. Res. Commun. 399: 429-433
2010
Cholesterol Depletion Mimics the Effect of Cytoskeletal Destabilization on Membrane Dynamics of the Serotonin1A Receptor: A zFCS Study
Biophys. J. 99:1397-1407
2010
Exploring tryptophan dynamics in acid-induced molten globule state of bovine a-lactalbumin: a wavelength-selective fluorescence approach
Eur Biophys J 39:1453-1463
2010
Membrane Cholesterol in the Function and Organization of G-protein Coupled Receptors in Cholesterol Binding and Cholesterol Transport Proteins: Structure and Function in Health and Disease
Subcellular Biochemistry series (Vol. 51, Harris, J.R., Ed.), Springer, New York, pp. 439-466.
2010
Membrane interaction of the N-terminal domain of chemokine receptor CXCR1
Biochimica et Biophysica Acta,1798: 1056-1061
2010
Monitoring Membrane Protein Conformational Heterogeneity by Fluorescence Lifetime Distribution Analysis Using the Maximum Entropy Method
J. Fluoresc, 20:407-413
2010
Chronic Cholesterol Depletion Using Statin Impairs the Function and Dynamics of Human Serotonin1A Receptors
Biochemistry, 49: 5426-5435
2010
Organization and dynamics of tryptophans in the molten globule state of bovine alpha-lactalbumin utilizing wavelength-selective ?uorescence approach: Comparisons with native and denatured states
Biochemical and Biophysical Research Communications, 394:1082-1086
2010
Metabolic Depletion of Sphingolipids Impairs Ligand Binding and Signaling of Human Serotonin1A Receptors
Biochemistry, 49:2389-2397
2010
Synthesis and Characterization of Environment-Sensitive Fluorescent Ligands for Human 5-HT1A Receptors with 1-Arylpiperazine Structure.
J. Med. Chem., 52: 7892-7896
2009
Organization and dynamics in micellar structural transition monitored by pyrene fluorescence.
Biochemical and Biophysical Research Communications, 390:728-732
2009
Membrane cholesterol depletion enhances ligand binding function of human serotonin1A receptors in neuronal cells
Biochemical and Biophysical Research Communications,390: 93-96
2009
Differential effects of cholesterol and desmosterol on the ligand binding function of the hippocampal serotonin1A receptor: Implications in desmosterolosis
Biochimica et Biophysica Acta, 1788: 2169-2173
2009
Membrane cholesterol depletion from live cells enhances the function of human serotonin1A receptors
Biochemical and Biophysical Research Communications,389:333-337
2009
Jagadish Chandra Bose: The First Indian Biophysicist
Asian Agri-History, 13:315-319
2009
Differential dynamics of membrane proteins in yeast
Biochemical and Biophysical Research Communications,387:661-665
2009
Effect of capsaicin on ligand binding activity of the hippocampal serotonin1A receptor
Glycoconj J,26:733-738
2009
The function of G-protein coupled receptors and membrane cholesterol: specific or general interaction?
Glycoconj J, 26:711-720
2009
Green ?uorescent protein: a molecular lantern that illuminates the cellular interior
J. Biosci.,34(2):169-172
2009
Orientation and Dynamics of a Novel Fluorescent Cholesterol Analogue in Membranes of Varying Phase
J. Phys.Chem. B,113:4475-4481
2009
The Green Journey
Journal of Fluorescence, 19: 1-2
2009
Are specific nonannular cholesterol binding sites present in G-protein coupled receptors?
Biochimica et Biophysica Acta, 1788: 295-302
2009
Monitoring Orientation and Dynamics of Membrane-Bound Melittin Utilizing Dansyl Fluorescence
J. Phys. Chem. B., 112: 14075-14082
2008
Effect of sphingomyelinase treatment on ligand binding activity of human serotonin1A receptors
Biochimica et Biophysica Acta, 1778:2022-2025
2008
Exploring the Organization and Dynamics of Hippocampal Membranes Utilizing Pyrene Fluorescence
J. Phys. Chem. B., 112:12134-12138
2008
Role of Tryptophan Residues in Gramicidin Channel Organization and Function
Biophysical Journal, 95:166-175
2008
Actin Cytoskeleton-Dependent Dynamics of the Human Serotonin1A Receptor Correlates with Receptor Signaling
Biophysical Journal, 95:451-463
2008
Signalling by the human serotonin1A receptor is impaired in cellular model of Smith-Lemli-Optiz Syndrome
Biochimica et Biophysica Acta- Biomembranes, 1778:1508-1516
2008
Differential Effects of Cholesterol and its Immediate Biosynthetic Precursors on Membrane Organization
Biochemistry, 47:5668-5677
2008
Membrane Organization and Function of the Serotonin1A Receptor
Cellular and Molecular Neurobiology, 27:1097-1116
2007
Dipolar Relaxation within the Protein Matrix of the Green fluorescent Protein: a Red Edge Excitation Shift Study
J. Phys. Chem. B., 111:14436-14439
2007
Differential effects of cholesterol and 7-dehydrocholesterol on ligand binding of solubillized hippocampal serotonin1A receptors: Implications in SLOS
Biochemical and Biophysical Research Communications., 363:800-805
2007
Solubilization of human serotonin1A receptors expressed in neuronal cells
Chemistry and Physics of Lipids., 150:244-249
2007
Dynamics and heterogeneity of bovine hippocampal membranes: Role of cholesterol and proteins
Biochimica et Biophysica Acta., 1768:2130-2144
2007
The gramicidin ion channel: A model membrane protein
Biochimica et Biophysica Acta.,1768: 2011-2025
2007
Melittin: a Membrane-active peptide with Diverse Functions
Biosci Rep., 27:189-223
2007
Effect of structural transition of the host assembly on dynamics of a membrane-bound tryptophan analogue
Biophysical Chemistry., 129:172-180
2007
Differential effects of cholesterol and 7-dehydrcholesterol on the ligand binding activity of the hippocampal serotonin1A receptor: Implications in SLOS
Biochemical and Biophysical Reserarch Communications., 358:495-499
2007
Membrane Organization of the Serotonin1A Receptor Monitored by a Detergent-Free Approach
Cellular and Molecular Neurobiology., 27(4):463-474
2007
Wavelength-selective fluorescence in ion channels formed by gramicidin A in membranes
J.Chem.Sci. 119:135-140c
2007
Monitoring the looping up of acyl chain labeled NBD lipids in membranes as a function of membrane phase state
Biochimica. et Biophysica Acta - Biomembranes 1768:1258-1267
2007
Monitoring the looping up of acyl chain labeled NBD lipids in membranes as a function of membrane phase state
Biochimica. et Biophysica Acta - Biomembranes 1768:1258-1267
2007
Modulation of gramicidin channel conformation and organization by hydrophobic mismatch in saturated phosphatidylcholine bilayers
Biochimica. et Biophysica Acta - Biomembranes 1768:1103-1113
2007
Influence of cholesterol and ergosterol on membrane dynamics using different fluorescent reporter probes
Biochem. Biophys. Res. Comm. 56:705-710
2007
Cholesterol: a potential therapeutic target in Leishmania infection
Trends Parasitol. 23:49-53
2007
Organization and Dynamics of NBD-Labeled Lipids in Membranes Analyzed by Fluorescence Recovery after Photobleaching
J. Phys. Chem. B , 111:1975-1983
2007
The human serotonin1A receptor exhibits G-protein-dependent cell surface dynamics
Glycoconj J 24:25-31
2007
Orientation and Dynamics of Melittin in Membranes of Varying Composition Utilizing NBD Fluorescence
Biophysical Journal 92:1271-1283
2007
Lipid-protein interactions, regulation and dysfunction of brain cholesterol
Biochemical and Biophysical Research Communications 354:627-633
2007
Cholesterol depletion induces dynamic confinement of the G-protein coupled serotonin1A receptor in the plasma membrane of living cells
Biochimica et Biophysica Acta - Biomembrane 1768:655-668
2007
Membrane localization and dynamics of Nile Red: Effect of cholesterol
Biochimica et Biophysica Acta - Biomembrane, 1768:59-66
2007
Spectrin Organization and Dynamics: New Insights
Biosci Rep 26:369-386
2006
The Human Serotonin 1A Receptor Expressed in Neuronal Cells: Toward a Native Environment for Neuronal Receptors
Cell. Mol. Neurobiol., 26: 925-942
2006
Effect of ionic strength on the organization and dynamics of membrane-bound melittin
Biophysical Chemistry,124: 115-124
2006
Monitoring the organization and dynamics of bovine hippocampal membranes utilizing differentially localized fluorescent membrane probes
Molecular Membrane Biology, 23(5): 430-441
2006
Effect of Ionic Strength on Folding and Aggregation of the Hemolytic Peptide Melittin in Solution
Biopolymers 83: 111-121
2006
Membrane interfacial localization of aromatic amino acids and membrane protein function
J. Biosci., 31(3): 297-302
2006
Effect of cholesterol on lateral diffusion of fluorescent lipid probes in native hippocampal membranes
Chemistry and Physics of Lipids 143: 11-21
2006
The Sterol-Binding Antibiotic Nystatin Inhibits Entry of Non-opsonized Leishmania donovani into Macrophages
Biochem. Biophys. Res. Commun., 339:661-666
2006
Monitoring ion channel conformations in membranes utilizing a novel dual fluorescence quenching approach
Biochem. Biophys. Res. Commun.,343:483-488
2006
Prolonged Treatment with Ligands Affects Ligand Binding to the Human Serotonin 1A Receptor in Chinese Hamster Ovary Cells
Cell. Mol. Neurobiol.,26(3): 247-257
2006
Role of Cholesterol in the Function and Organization of G-protein Coupled Receptors
Progress in Lipid Research, 45: 295-333
2006
Confocal Fluorescence Recovery After Photobleaching of Green Fluorescent Protein in Solution
J. Fluorescence, 16: 87-94
2006
Ligand Binding and G-protein Coupling of the Serotonin 1A Receptor in Cholesterol-enriched Hippocampal Membranes
Bioscience Rep., 26:79-87
2006
aB-crystallin, a small heat-shock protein, prevents the amyloid fibril growth of an amyloid ?peptide and ?-microglobulin
Biochemical Journal., 392: 573-581
2005
The Serotonin1A Receptor and its Interaction with the Membrane Environment
Proc. Indian Natl. Sci. Acad. B71 (2): 61-72
2005
Membrane Protein Solubilization: Recent Advances and Challenges in Solubilization of Serotonin 1A Receptors
IUBMB Life, 57:505-512
2005
Membrane Cholesterol Oxidation Inhibits Ligand Binding Function of Hippocampal Serotonin 1A Receptors
Biochem. Biophys. Res. Commun. 331:422-427
2005
The Serotonin 1A Receptor: A Representative Member of the Serotonin Receptor Family
Cell. Mol. Neurobiol. 25: 553-580
2005
Membrane organization of the human serotonin 1A receptor monitored by detergent insolubility using GFP fluorescence
Mol. Membr. Biol., 22:539-547
2005
Effect of Structural Transition of the Host Assembly on Dynamics of an Ion Channel Peptide: A Fluorescence Approach
Biophys. J., 89:3049-3058
2005
Novel Insights Into Protein Structure and Dynamics Utilizing the Red Edge Excitation Shift Approach
Reviews in Fluorescence, pp. 199-222
2005
Membrane Organization and Dynamics of the G-protein Coupled Serotonin 1A Receptor Monitored using Fluorescence-based Approaches
J. Fluoresc.15:785-796
2005
Monitoring the organization and dynamics of bovine hippocampal membranes utilizing Laurdan generalized polarization .
Biochim. Biophys. Acta - Biomembrane 1714: 43-55.
2005
Cholesterol Modulates the Antagonist Binding Funtion of Hippocampal Serotonin 1A Receptors
Biochim. Biophys. Acta - Biomembrane 1714: 35-42.
2005
The cholesterol-complexing agent digitonin modulates ligand binding of the bovine hippocampal serotonin 1A receptor
Mol. Membr. Biol. 22: 241-249.
2005
Ion Channels and d-amino acids
J. Biosci. 30: 147-149.
2005
Effect of Ionic Strength on the Organization and Dynamics of Tryptophan Residues in Spectrin: A Fluorescence Approach
Biopolymers 77: 325-334
2005
Cholesterol inhibits the lytic activity of melittin in erythrocytes
Chem. Phys. Lipids 134: 183-189
2005
Monitoring cholesterol organization in membranes at low concentrations utilizing the wavelength-selective fluorescence approach
Chem. Phys. Lipids 134: 79-84
2005
Influence of Ester and Ether Linkage in Phospholipids on the Environment and Dynamics of the Membrane Interface: A Wavelength-Selective Fluorescence Approach
Langmuir 21: 287-293
2005
Effect of Graded Hydration on the Dynamics of an Ion Channel Peptide: A Fluorescence Approach
Biophys. J. 88:1070-1080
2005
Role of cholesterol in ligand binding and G-protein coupling of Serotonin 1A receptors solubilized from bovine hippocampus
Biochem. Biophys.Res. Commun. 327: 1036-1041
2005
Heat stress-induced localization of small heat shock proteins in mouse myoblasts: intranuclear lamin a/c speckles as target for alhpaB-crystallin and hsp25
Experimental Cell Research ., 299: 393-403
2004
Depth-Dependent Solvent Relaxation in Reverse Micelles: A Fluorescence Approach
J. Phys. Chem. B 108: 12151-12158
2004
Ligand BindingCharacteristics of the Human Serotonin 1A Receptor Heterologously Expressed in CHO Cells
Biosci. Rep. 24, 101-115.
2004
G-protein-dependent Cell Surface Dynamics of the Human Serotonin 1A Receptor Tagged to Yellow Fluorescent Protein
Biochemistry 43: 15852-15862
2004
Effect of Micellar Charge on the Conformation and Dynamics of Melittin
Effect of Micellar Charge on the Conformation and Dynamics of Melittin
2004
A GFP Fluorescence-based Approach to Determine Detergent Insolubility of the Human Serotonin 1A Receptor
FEBS Lett. 576: 455-460
2004
Effect of Mg 2+ on Guanine Nucleotide Sensitivity of Ligand Binding to Serotonin 1A Receptors from Bovine Hippocampus
Biochem. Biophys.Res. Commun. 323: 372-376
2004
Interaction of Melittin with Membrane Cholesterol: A Fluorescence Approach
Biophys. J.87: 2419-2432
2004
Monitoring Gramicidin Conformations in Membranes: A Fluorescence Approach
Biophys. J.87: 831-843
2004
Influence of Lipid Chain Unsaturation on Membrane-Bound Melittin: A Fluorescence Approach
Biochim. Biophys. Acta - Biomembrane 1665: 29-39
2004
The Sterol-Binding Antibiotic Nystatin Differentially Modulates Agonist and Antagonist Binding of the Bovine Hippocampal Serotonin 1A Receptor
Biochem. Biophys.Res. Commun. 320: 557-562
2004
Influence of Cholesterol and Ergosterol on Membrane Dynamics: A Fluorescence Approach
Biochem. Biophys.Res. Commun. 318: 920-926
2004
Cholesterol Modulates Ligand Binding and G-protein Coupling to Serotonin 1A Receptors from Bovine Hippocampus
Biochim. Biophys. Acta - Biomembrane 1663: 188-200
2004
Membrane Sphingolipid-Ergosterol Interactions are Important Determinants of Multi Drug Resistance in Candida albicans
Antimicrob. AgentsChemother. 48: 1778-1787
2004
Interaction of Serotonin 1A Receptors from Bovine Hippocampus with Tertiary Amine Local Anesthetics
Cell. Mol. Neurobiol. 24: 403-422
2004
Serotonin 1A receptor agonist acquires an antimalarial connection
J. Biosci. 29: 101-102
2004
Temperature Dependent Interaction of the Bovine Hippocampal Serotonin 1A Receptor with G-proteins
Mol. Membr. Biol. 21: 119-123
2004
Effect of Urea on the Organization and Dynamics of Triton X-100 Micelles: A Fluorescence Approach
J. Phys. Chem. B 108: 2489-2496
2004
Exploring Detergent Insolubility in Bovine Hippocampal Membranes: A Critical Assessment of the Requirement for Cholesterol
Biochim. Biophys. Acta - Biomembrane 166: 9-17
2004
Cholesterol is Required for Leishmania donovani Infection: Implications in Leishmaniasis
Mol.Biochem. Parasitol. 133: 145-152
2004
Organization and Dynamics of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled Lipids: A Fluorescence Approach
Chem. Phys. Lipids 127: 91-101
2004
Solubilization of Serotonin 1A Receptors Heterologously Expressed in Chinese Hamster Ovary Cells
Cell. Mol. Neurobiol. 24: 293-300
2004
Application of fluorescence spectroscopy to membrane protein structure and dynamics
Current Science, Vol.87, No.2.
2004
Organization and Dynamics of Melittin in Environments of Graded Hydration: A Fluorescence Approach
Langmuir 19: 10332-10341
2003
Organization and Dynamics of Tryptophan Residues in Erythroid Spectrin: Novel Structural Features of Denatured Spectrin Revealed by the Wavelength-SelectiveFluorescence Approach
Protein Sci. 12: 2389-2403
2003
Modulation of Fluorophore Environment in Host Membranes of Varying Charg
J. Fluoresc. 13: 459-466
2003
Modulation of Fluorophore Environment in Host Membranes of Varying Charg
J. Fluoresc. 13: 459-466
2003
Modulation of Fluorophore Environment in Host Membranes of Varying Charg
J. Fluoresc. 13: 459-466
2003
Modulation of Fluorophore Environment in Host Membranes of Varying Charg
J. Fluoresc. 13: 459-466
2003
Modulation of Fluorophore Environment in Host Membranes of Varying Charg
J. Fluoresc. 13: 459-466
2003
Modulation of Fluorophore Environment in Host Membranes of Varying Charg
J. Fluoresc. 13: 459-466
2003
Novel Insights into Membrane Protein Structure and Dynamics utilizing the Wavelength-Selective Fluorescence Approach
Novel Insights into Membrane Protein Structure and Dynamics utilizing the Wavelength-Selective Fluorescence Approach
2003
Exploring Membrane Organization and Dynamics by the Wavelength-Selective Fluorescence Approach
Chem. Phys. Lipids 122: 3-17
2003
Reverse Micellar Organization and Dynamics: A Wavelength-Selective Fluorescence Approach
J. Phys. Chem. B 106: 13002-13009
2002
Application of the Wavelength-selective Fluorescence Approach to Monitor Membrane Organization and Dynamics
Fluorescence Spectroscopy, Imaging and Probes(Kraayenhof, R., Visser, A.J.W.G., and Gerritsen, H.C., Eds.), pp. 211-224, Springer-Verlag, Heidelberg
2002
Solubilization of High Affinity G-Protein-Coupled Coupled Serotonin 1A Receptors from Bovine Hippocampus using Pre-Micellar CHAPS at Low Concentration
Mol. Membr. Biol. 19: 211-220
2002
Modulation of Antagonist Binding to Coupled Serotonin 1A Receptors from Bovine Hippocampus by Metal Ions
Cell. Mol. Neurobiol. 21: 453-464
2001
Cholesterol Organization in Membranes at Low Concentrations: Effects of Curvature Stress and Membrane Thickness
Biophys. J. 81: 2122-2134
2001
Effect of Alcohols on G-Protein Coupling of Coupled Serotonin 1A Receptors from Bovine Hippocampus
Brain Res. Bull. 52: 597-601
2000
A New Paradigm in the Functioning of G-Protein-Coupled Receptors
Curr. Sci. 79: 402-403
2000
Role of Disulfides and Sulfhydryl Groups in Agonist and Antagonist Binding in Serotonin 1A Receptors from Bovine Hippocampus
Cell. Mol. Neurobiol. 20: 665-681
2000
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